TRT with Free Testosterone Blood Test
    TRT with Free Testosterone Blood Test
    TRT with Free Testosterone Blood Test
    TRT with Free Testosterone Blood Test

TRT with Free Testosterone Blood Test


Monitor your hormones with our advanced TRT hormone monitoring profile, including free testosterone, oestradiol, and prolactin.

Results estimated in 2 working days

View 6 Biomarkers

How do you want to take your sample?

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  • Book a venous draw at a clinic   +£35.00

    Visit one of our national clinic partners for a nurse to take your venous blood sample from a vein in your arm. We’ll email you instructions on how to book after we’ve processed your order.
  • Book a venous draw at home with a nurse +£59.00

  • Self-arrange a professional sample collection Free

TRT with Free Testosterone Blood Test

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Is it for you?

Are you taking testosterone replacement therapy and want a thorough monitoring profile that includes free testosterone, prolactin, and oestradiol?

Our monitoring profile with free testosterone has been developed with a specialist TRT doctor and is perfect if you want to monitor your hormones whilst taking TRT.

Biomarker table



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Oestradiol is a female steroid hormone, produced in the ovaries of women and to a much lesser extent in the testes of men. It is the strongest of three oestrogens and is responsible for the female reproductive system as well as the growth of breast tissue and bone thickness. In pre-menopausal women, oestradiol levels vary throughout the monthly cycle, peaking at ovulation. In women, oestradiol levels decline with age, culminating with the menopause when the ovaries stop producing eggs. Low oestradiol can cause many symptoms associated with the menopause, including hot flushes, night sweats and mood swings. Low oestradiol can also cause osteoporosis.


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Testosterone is a hormone that causes male characteristics. For men, it helps to regulate sex drive and has a role in controlling bone mass, fat distribution, muscle mass, strength and the production of red blood cells and sperm. Testosterone is produced in the testicles of men and, in much smaller amounts, in the ovaries of women. Testosterone levels in men naturally decline after the age of 30, although lower than normal levels can occur at any age and can cause low libido, erectile dysfunction, difficulty in gaining and maintaining muscle mass and lack of energy. Although women have much lower amounts of testosterone than men, it is important for much the same reasons, playing a role in libido, the distribution of muscle and fat and the formation of red blood cells. All laboratories will slightly differ in the reference ranges they apply because they are based on the population they are testing. The normal range is set so that 95% of men will fall into it. For greater consistency, we use the guidance from the British Society for Sexual Medicine (BSSM) which advises that low testosterone can be diagnosed when testosterone is consistently below the reference range, and that levels below 12 nmol/L could also be considered low, especially in men who also report symptoms of low testosterone or who have low levels of free testosterone.

Free testosterone - calc

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Most testosterone circulating in the blood is bound to proteins, in particular SHBG and albumin; only 2-3 % of testosterone is free and available to cells. This test uses an algorithm to calculate the level of free or unbound testosterone in relation to total testosterone, SHBG and albumin.


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Prolactin is a hormone which is produced in the pituitary gland and plays a role in reproductive health. Its primary purpose is to stimulate milk production after childbirth, and in pregnant and breastfeeding women prolactin levels can soar.



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Albumin is a protein which is made mainly in the liver. It helps to exert the osmotic pressure which holds water within the blood. It also helps carry nutrients and medications and other substances through the blood and is important for tissue growth and healing. Albumin also carries hormones around the body, therefore measuring the amount of albumin in the blood can help us calculate how much hormone is available to your tissues.


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SHBG (sex hormone binding globulin) is a protein which transports the sex hormones (testosterone, oestrogen and dihydrotestosterone (DHT)) in the blood.Hormones which are bound to SHBG are inactive which means that they are unavailable to your cells. Measuring the level of SHBG in your blood gives important information about your levels of free or unbound hormones which are biologically active and available for use.
Special instructions

How to prepare for your test

Prepare for your TRT with Free Testosterone Blood Test by following these instructions. Take your sample between 6am and 10am. Avoid nipple stimulation or any kind of sexual activity for 24 hours before taking this test, as these can increase prolactin levels. Heavy meals and strenuous exercise can also impact prolactin results and are best avoided before taking the test. Do not take biotin supplements for two days before this test, discuss this with your doctor if it is prescribed.

Blood testing made easy

How it works

Your personalised, actionable health results are only a few clicks away. Order your test, take and post your sample, then view your results online with our doctors' comments.

Your results, simplified

Track, improve, and monitor your health over time

MyMedichecks is your personal online dashboard where you can view your results, access clear and simple explanations about individual health markers, monitor changes in your health, and securely store information about your medical history, lifestyle, and vital statistics.


What can I learn from a TRT with free testosterone test?

Our test includes total testosterone and a calculation of the amount of free or unbound testosterone that is available to your tissues. Most testosterone in your body is bound to proteins (sex hormone-binding globulin and albumin) and is unavailable to your cells. By measuring your total testosterone and your levels of SHBG and albumin, the laboratory can calculate your free testosterone level. If you have high levels of SHBG, your free testosterone will be lower, even when your total testosterone is normal.

Why check free testosterone and female hormones?

Low free testosterone can cause low libido, lack of muscle mass, and low mood. It is also important to check the levels of female hormones in your blood if you are taking TRT. High testosterone levels can also cause high oestradiol, especially if you are experiencing symptoms associated with high oestradiol (including the growth of breast tissue and low libido).

Who is a TRT with free testosterone test for?

Our TRT with Free Testosterone Blood Test profile is for men taking testosterone replacement therapy (TRT) who want to monitor their male and female hormones. Monitoring your hormone levels allows you and your practitioner to optimise your dose of testosterone replacement. This test is also useful if you are experiencing symptoms of low testosterone yet your total testosterone level is normal, and you want to measure your free testosterone as the next step.

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