Monitor your hormone levels with our TRT hormone monitoring profile, which includes free testosterone, oestradiol, and prolactin, to ensure your treatment stays on track.
TRT with Free Testosterone Blood Test
£81
- Results estimated in 2 working days
- Convenient testing options
- Bespoke doctor’s report
- Free delivery
Take your monitoring a step further with our TRT Blood Test. It not only checks testosterone, oestradiol, and prolactin but also includes red blood cell count and prostate health markers to give a more complete view of your hormone balance, treatment response, and potential side effects.
How do you want to take your sample?
Please choose one option below-
Book a venous draw at a clinic Venous+£35Visit one of our national clinic partners for a nurse to take your venous blood sample from a vein in your arm. We’ll email you instructions on how to book after we’ve processed your order.
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Book a venous draw at home with a nurse Venous+£59
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Self-arrange a professional sample collection VenousFree
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Your results and next steps
What could my results show?
Your results will be available in your MyMedichecks account — your secure online dashboard with clear explanations, health trends, and medical history storage.
If your testosterone is low, the British Society of Sexual Medicine recommends a second, more in-depth test after four weeks.
Low T? We know someone.
Leger – specialists in low T
If your results show low testosterone, Leger can help. Their expert team specialises in testosterone treatment, offering safe, effective care tailored to you.
If you've got a low testosterone result, Leger can advise on the next steps, whether that's a second test or a consultation with a TRT specialist.
Low energy
Low sex drive
Mood changes
Low muscle mass
Poor sleep quality
Lack of motivation
Erectile dysfunction
More body fat
How do venous blood tests work?
Order your test
Find the right test for you and order online. Book a nurse visit at home, visit a partner clinic, or arrange your own blood draw. We’ll send you everything you need to get started.
Take your sample
A nurse or phlebotomist will collect your sample safely and professionally. Your sample is then sent to our accredited laboratory for expert analysis.
Get your results
Your results will be uploaded securely to your account with clear explanations alongside from one of our doctors, so you know exactly what they mean.
What's in the test?
Hormones
Oestradiol
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Oestradiol is the strongest of the three oestrogens. It's labelled a female hormone but it's made in both the ovaries and testes. It's responsible for the growth of breast tissue, the female reproductive system, and male sexual function.
In pre-menopausal women, oestradiol levels vary throughout the monthly cycle, peaking just before ovulation. Levels are lowest after menopause when the ovaries stop producing eggs. Low oestradiol levels in women can cause many symptoms associated with the menopause, including hot flushes, night sweats, and changes in mood. It can also increase the risk of osteoporosis (a condition where the bones become less dense).
In men, oestradiol levels tend to increase slightly with age as testosterone levels decline. Too much oestradiol in men can contribute to fertility problems, gynecomastia (enlarged breast tissue), and erectile dysfunction.
Testosterone
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Testosterone is a hormone that causes male characteristics. For men, it helps to regulate sex drive and has a role in controlling bone mass, fat distribution, muscle mass, strength and the production of red blood cells and sperm. Testosterone is produced in the testicles of men and, in much smaller amounts, in the ovaries of women. Testosterone levels in men naturally decline after the age of 30, although lower than normal levels can occur at any age and can cause low libido, erectile dysfunction, difficulty in gaining and maintaining muscle mass and lack of energy. Although women have much lower amounts of testosterone than men, it is important for much the same reasons, playing a role in libido, the distribution of muscle and fat and the formation of red blood cells. All laboratories will slightly differ in the reference ranges they apply because they are based on the population they are testing. The normal range is set so that 95% of men will fall into it. For greater consistency, we use the guidance from the British Society for Sexual Medicine (BSSM) which advises that low testosterone can be diagnosed when testosterone is consistently below the reference range, and that levels below 12 nmol/L could also be considered low, especially in men who also report symptoms of low testosterone or who have low levels of free testosterone.
Free testosterone - calc
Learn more
Most testosterone circulating in the blood is bound to proteins, in particular SHBG and albumin; only 2-3 % of testosterone is free and available to cells. This test uses an algorithm to calculate the level of free or unbound testosterone in relation to total testosterone, SHBG and albumin.
Prolactin
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Prolactin is a hormone which is produced in the pituitary gland and plays a role in reproductive health. Its primary purpose is to stimulate milk production after childbirth, and in pregnant and breastfeeding women prolactin levels can soar.
Proteins
Albumin
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Albumin is a protein which is made mainly in the liver. It helps to exert the osmotic pressure which holds water within the blood. It also helps carry nutrients and medications and other substances through the blood and is important for tissue growth and healing. Albumin also carries hormones around the body, therefore measuring the amount of albumin in the blood can help us calculate how much hormone is available to your tissues.
SHBG
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SHBG (sex hormone binding globulin) is a protein which transports the sex hormones (testosterone, oestrogen and dihydrotestosterone (DHT)) in the blood.Hormones which are bound to SHBG are inactive which means that they are unavailable to your cells. Measuring the level of SHBG in your blood gives important information about your levels of free or unbound hormones which are biologically active and available for use.
How to prepare for your test
Prepare for your TRT with Free Testosterone Blood Test by following these instructions.
Sample collection and timing
- Take your sample between 6am and 10am.
- Avoid vigorous exercise or sexual activity for 48 hours beforehand, as these can raise your prolactin level.
- Avoid nipple stimulation or heavy meals before taking the test, as these can increase prolactin levels.
- For tests that include prolactin, take your sample before 10 am and at least an hour after waking.
Medications and supplements
- Do not take biotin supplements for two days before this test, discuss this with your doctor if it is prescribed.
Test limitations
Reviews
FAQs
What can I learn from a TRT with free testosterone test?
Our test includes total testosterone and a calculation of the amount of free or unbound testosterone that is available to your tissues. Most testosterone in your body is bound to proteins (sex hormone-binding globulin and albumin) and is unavailable to your cells. By measuring your total testosterone and your levels of SHBG and albumin, the laboratory can calculate your free testosterone level. If you have high levels of SHBG, your free testosterone will be lower, even when your total testosterone is normal.
Why check free testosterone and female hormones?
Low free testosterone can cause low libido, lack of muscle mass, and low mood. It is also important to check the levels of female hormones in your blood if you are taking TRT. High testosterone levels can also cause high oestradiol, especially if you are experiencing symptoms associated with high oestradiol (including the growth of breast tissue and low libido).
Who is a TRT with free testosterone test for?
Our TRT with Free Testosterone Blood Test profile is for men taking testosterone replacement therapy (TRT) who want to monitor their male and female hormones. Monitoring your hormone levels allows you and your practitioner to optimise your dose of testosterone replacement. This test is also useful if you are experiencing symptoms of low testosterone yet your total testosterone level is normal, and you want to measure your free testosterone as the next step.
Why have the male reference ranges for testosterone changed?
The reference range for testosterone has been adjusted to have a lower limit of 12 nmol/L to reflect guidance from the British Society for Sexual Medicine (BSSM) 2022.
Our medical advice regarding testosterone levels remains the same and we advise that if your total testosterone level is between the range of 8-12 nmol/L and you have symptoms of low testosterone you may be considered eligible for testosterone replacement therapy. If your testosterone level falls within this range but you have no symptoms this is considered satisfactory and no further action is required.
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