Day 3 Fertility Blood Test, from our experts to you.
Dr Sam Rodgers MBBS, MRCGP

Chief Medical Officer meet our doctors

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What is a
fertility blood test?

Our day three fertility test is a helpful first test in an investigation into female infertility. Ideally, you should take our test three days after the start of your period, but it can also be taken on days two, four, or five of your menstrual cycle. This test aims to check that hormone levels can support the maturation and release of a healthy egg from the ovary.


What can I learn
from this test?

Our test includes Follicle-stimulating hormone (FSH), luteinising hormone (LH), and oestradiol. FSH is released from a gland in your brain (the pituitary gland) to stimulate the ovary to start maturing an egg. LH is required for the final maturation and release of the egg from a follicle (a fluid-filled sac containing the egg), while the hormone oestradiol stimulates the growth of the follicle and prepares the womb lining for pregnancy.

The levels of hormones in your body on day three of your cycle can indicate whether hormonal problems are likely to affect ovulation and conception and whether a condition like polycystic ovaries may be affecting fertility.


What could
affect my results?

Please note that any hormones you are taking for contraception or hormone replacement therapy (HRT) will influence your results. If you are looking for a true picture of your fertility status then please take this test at least 12 weeks after stopping any hormone replacement or contraceptive pill, implant or hormone IUD. It is recommended to wait until your periods have re-established a regular cycle (for you) before taking this test.


What's included?

Hormones
Select profile for more information

Hormone phase Hormone phase is used to determine the appropriate range for hormone markers and is calculated using the date of last menstrual period and the date of the sample.
FSH Follicle Stimulating Hormone (FSH) is produced in the pituitary gland and is important for women in the production of eggs by the ovaries and for men for men in the production of sperm. In the first half of the menstrual cycle in women, FSH stimulates the enlargement of follicles within the ovaries. Each of these follicles will help to increase oestradiol levels. One follicle will become dominant and will be released by the ovary (ovulation), after which follicle stimulating hormone levels drop during the second half of the menstrual cycle. In men, FSH acts on the seminiferous tubules of the testicles where they stimulate immature sperm cells to develop into mature sperm.
LH Luteinising Hormone (LH) is produced by the pituitary gland and is important for male and female fertility. In women it governs the menstrual cycle, peaking before ovulation. In men it stimulates the production of testosterone.
Oestradiol Oestradiol is a female steroid hormone, produced in the ovaries of women and to a much lesser extent in the testes of men. It is the strongest of three oestrogens and is responsible for the female reproductive system as well as the growth of breast tissue and bone thickness. In pre-menopausal women, oestradiol levels vary throughout the monthly cycle, peaking at ovulation. In women, oestradiol levels decline with age, culminating with the menopause when the ovaries stop producing eggs. Low oestradiol can cause many symptoms associated with the menopause, including hot flushes, night sweats and mood swings. Low oestradiol can also cause osteoporosis.

How to prepare
for your test

Special instructions

Prepare for your Day 3 Fertility Blood Test by following these instructions. Take this test two to five days after the start of your period, ideally on day three. It can be taken any time if you do not have periods. Hormonal contraception can affect this test, taking a break from this and using barrier contraception will give more accurate results. Avoid taking your sample from a finger used to apply hormone gels/pessaries in the past 4 weeks. Use gloves to apply these. Do not take biotin supplements for two days before this test, discuss this with your doctor if it is prescribed.


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