Day 3 Fertility Blood Test
    Day 3 Fertility Blood Test
    Day 3 Fertility Blood Test
    Day 3 Fertility Blood Test
    Day 3 Fertility Blood Test

Day 3 Fertility Blood Test


Measure your reproductive hormones to get an idea of your ovarian reserve and egg production.

Results estimated in 2 working days

View 3 Biomarkers

How do you want to take your sample?

Please choose one option below
  • Collect your own finger-prick blood sample at home   Free

    We’ll send you everything you need to collect your blood sample from your finger at home.
  • Book a venous draw at a clinic   +£35.00

  • Book a venous draw at home with a nurse +£59.00

  • Self-arrange a professional sample collection Free

Day 3 Fertility Blood Test

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Is it for you?

Are you looking for answers about your fertility? Take our Day 3 Fertility Blood Test on the third day of your period to give you an idea of your ovarian reserve. It can be a helpful test if you are currently trying or thinking about starting a family and would like to check your reproductive hormones.

Biomarker table



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Follicle Stimulating Hormone (FSH) is produced in the pituitary gland and is important for women in the production of eggs by the ovaries and for men in the production of sperm. In the first half of the menstrual cycle in women, FSH stimulates the enlargement of follicles within the ovaries. Each of these follicles will help to increase oestradiol levels. One follicle will become dominant and will be released by the ovary (ovulation), after which follicle stimulating hormone levels drop during the second half of the menstrual cycle. In men, FSH acts on the seminiferous tubules of the testicles where they stimulate immature sperm cells to develop into mature sperm.


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Luteinising Hormone (LH) is produced by the pituitary gland and is important for male and female fertility. In women it governs the menstrual cycle, peaking before ovulation. In men it stimulates the production of testosterone.


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Oestradiol is a female steroid hormone, produced in the ovaries of women and to a much lesser extent in the testes of men. It is the strongest of three oestrogens and is responsible for the female reproductive system as well as the growth of breast tissue and bone thickness. In pre-menopausal women, oestradiol levels vary throughout the monthly cycle, peaking at ovulation. In women, oestradiol levels decline with age, culminating with the menopause when the ovaries stop producing eggs. Low oestradiol can cause many symptoms associated with the menopause, including hot flushes, night sweats and mood swings. Low oestradiol can also cause osteoporosis.
Special instructions

How to prepare for your test

Prepare for your Day 3 Fertility Blood Test by following these instructions. Take this test two to five days after the start of your period, ideally on day three. It can be taken any time if you do not have periods. Avoid taking a finger-prick sample from a finger used to apply hormone gels/pessaries/patches in the past 4 weeks. Use gloves to apply these. Do not take biotin supplements for two days before this test, discuss this with your doctor if it is prescribed. Hormonal contraception can affect the results of this test. Taking a break from this and waiting for your periods to restart before your blood test will give more accurate results.

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How it works

Your personalised, actionable health results are only a few clicks away. Order your test, take and post your sample, then view your results online with our doctors' comments.

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What can I learn from this test?

Our test includes Follicle-stimulating hormone (FSH), luteinising hormone (LH), and oestradiol. FSH is released from a gland in your brain (the pituitary gland) to stimulate the ovary to start maturing an egg. LH is required for the final maturation and release of the egg from a follicle (a fluid-filled sac containing the egg), while the hormone oestradiol stimulates the growth of the follicle and prepares the womb lining for pregnancy.

The levels of hormones in your body on day three of your cycle can indicate whether hormonal problems are likely to affect ovulation and conception and whether a condition like polycystic ovaries may be affecting fertility.

What could affect my results?

Please note that any hormones you are taking for contraception or hormone replacement therapy (HRT) will influence your results. If you are looking for a true picture of your fertility status then please take this test at least 12 weeks after stopping any hormone replacement or contraceptive pill, implant or hormone IUD. It is recommended to wait until your periods have re-established a regular cycle (for you) before taking this test.

What is a fertility blood test?

Our day three fertility test is a helpful first test in an investigation into female infertility. Ideally, you should take our test three days after the start of your period, but it can also be taken on days two, four, or five of your menstrual cycle. This test aims to check that hormone levels can support the maturation and release of a healthy egg from the ovary.

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